Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Sample
Mouse Tissue lysate - whole (Dorsal Hippocampal Tissue)
Specification
Dorsal Hippocampal Tissue
Blocking step
I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Flu
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/1000
Detection
Detection method
Scanning Imager detecting at 647nm
Bands
Specific: 55 kDa Non-specific: 42, 50 kDa
Exposure
12 minute(s) and 36 second(s)
Negative control
N/A
Positive control
Dorsal Hippocampal Samples stored in RIPA Buffer after behavioral testing.
Additional data
Additional Notes
All sample lanes were Dorsal Hippocampal Samples from C57BL/6 mice stored in a RIPA buffer.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 14 2013