Application
Flow Cytometry
Sample
Human Cell (HeLa)
Specification
HeLa
Preparation
Cell harvesting/tissue preparation method: grown in TC
Sample buffer: harvested by scraping in cold PBS
Sample buffer: harvested by scraping in cold PBS
Fixation
BD CytoFix/CytoPerm
Permeabilization
Yes - BD CytoFix/CytoPerm
Gating Strategy
single cells
Other product details
Dilution
1/200
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: BD CytoPerm/CytoWash
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen A21206
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Cells stained with primary antibody had a signal two logs greater than negative control cells stained with secondary antibody only.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Lee Gehrke
Verified customer
投稿 Apr 10 2013