Anti-P4HA1 抗体 (ab88881)
Key features and details
- Mouse polyclonal to P4HA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-P4HA1 antibody
P4HA1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to P4HA1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human P4HA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- A431 whole cell lysate (ab7909) can be used as a positive control in WB. A-431 cell lysate and P4HA1 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88881の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. |
ターゲット情報
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機能
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. -
配列類似性
Belongs to the P4HA family.
Contains 1 Fe2OG dioxygenase domain.
Contains 1 TPR repeat. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 5033 Human
- SwissProt: P13674 Human
- Unigene: 500047 Human
- Unigene: 593005 Human
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別名
- 2-oxoglutarate-4-dioxygenase subunit alpha-1 antibody
- 4-hydroxylase, alpha I subunit antibody
- 4-PH alpha-1 antibody
see all
画像
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Anti-P4HA1 antibody (ab88881) at 1 µg/ml + A-431 cell lysate at 50 µg
Predicted band size: 61 kDa
Observed band size: 61 kDa -
All lanes : Anti-P4HA1 antibody (ab88881) at 1 µg/ml
Lane 1 : P4HA1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
データシートおよび資料
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Datasheet download
参考文献 (1)
ab88881 は 1 報の論文で使用されています。
- Dai L et al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics 11:4021-8 (2011). PubMed: 21898824