Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse bone cells)
Specification
mouse bone cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5%
Other product details
Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: chicken anti goat
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
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Abcam user community
Verified customer
投稿 Nov 24 2008