Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Calvarial Tissue)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
With Insulin as Positive Control
Specification
Mouse Calvarial Tissue
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat (polyclonal) anti-Rabbit IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Negative control
negative Controls are mouse calvariae untreated
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: Not detected kDa Non-specific: 24, 31, 45, 73 kDa
Positive control
Positive control are mouse calvariae treated with Insulin (100 ug/ml), This treatment is expected to increase the osteoblast activity and therefore the osteocalcin expression levels.
Additional data
Additional Notes
The antibody tested within this application is completely unsatisfactory. It does not detect the band of interest (11 kDa). Additionally, several non-specific bands are detected. This compromises the integrity of data interpretation for IHC and ICC analysis. Therefore, I would not recommend this antibody.
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 29 2016