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Full length protein corresponding to Cow Osteocalcin.
Database link: P02820
Though we have found ab13420 does not react with mouse sample in our lab, there is a report from a reviewer that got positive result with mouse sample in IHC-Fr. However, this information needs to be confirmed further.
Although this antibody does work in IHC-P, we have found that ab13418 typically gives a more robust signal. Therefore, if you plan on using this antibody in IHC-P, we recommend ab13418 as an alternative option.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab13420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 12 kDa.
Use under reducing or non-reducing conditions.
|IHC-Fr||Use a concentration of 1 - 10 µg/ml.|
|IHC-P||Use a concentration of 10 µg/ml. Enzymatic retrieval with Proteinase K is recommended.|
|Sandwich ELISA||Use a concentration of 1 - 10 µg/ml.
For use in sandwich ELISA with ab13421
|ICC/IF||Use at an assay dependent concentration. PubMed: 21396708|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"