Application
Immunohistochemistry (Frozen sections)
Blocking step
fish gelantine and ovalbumine as blocking agent for 30 minute(s) · Concentration: 0.6% · Temperature: 22°C
Sample
Mouse Tissue sections (Retina)
Specification
Retina
Permeabilization
Yes - PBS-Tween 0.01%
Fixative
only frozen
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/400
Additional data
Additional Notes
Staining in the connecting cilium of the mouse retina varies between GR52215-3 and GR52215-2. The staining of GR52215-2 was done several times and looked always identical. But since I use GR52215-3 I was unable to reproduce the results from GR52215.2.
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投稿 Jul 23 2013