Anti-Oligodendrocyte Specific Protein 抗体 - Oligodendrocyte Marker (ab7474)
Key features and details
- Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
Oligodendrocyte Specific Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker -
由来種
Rabbit -
特異性
Reacts specifically with 22 kDa protein derived from CNS samples. -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Rat, Human -
免疫原
A 15 residue synthetic C-terminal peptide.
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特記事項
The concentration depends on the batch, please enquire for current stock concentration.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7474の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (2) |
1/50 - 1/500.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
1/50 - 1/500. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5010 Human
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: O75508 Human
- SwissProt: Q99P82 Rat
- Unigene: 31595 Human
- Unigene: 8282 Rat
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別名
- Claudin 11 antibody
- Claudin-11 antibody
- CLD11_HUMAN antibody
see all
画像
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Immunohistochemical analysis of adult mouse testis tissue, staining Oligodendrocyte Specific Protein (red) with ab7474.
Tissue was blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA) for 1 hour at 25°C. A TRITC-conjugated donkey anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. -
Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
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IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
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Datasheet download
参考文献 (14)
ab7474 は 14 報の論文で使用されています。
- Valdivia AO & Bhattacharya SK Lyso-Lipid-Induced Oligodendrocyte Maturation Underlies Restoration of Optic Nerve Function. eNeuro 9:N/A (2022). PubMed: 35027445
- Wellman SM et al. Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces. Front Neurosci 13:493 (2019). PubMed: 31191216
- Raynald et al. Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats. CNS Neurosci Ther 25:951-964 (2019). PubMed: 31486601
- Pan J et al. Expression of claudin-11 in a rat model of varicocele and its effects on the blood-testis barrier. Mol Med Rep 18:5647-5651 (2018). PubMed: 30365105
- Zhao X et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. Nat Commun 8:602 (2017). PubMed: 28928459