Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 594-anti-Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
PubMed ID
22888021: View
Additional Notes
OR2A4 localizes to the spindle poles (red arrows) and chromosomes (green arrow) during mitosis and to the cleavage furrow (yellow arrow) and midbody ring/Flemming body (blue arrow in cell adjacent to metaphase cell) in cytokinesis.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 02 2012