Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Sample
Dog Tissue sections (MDCK pellets in paraffin)
Specification
MDCK pellets in paraffin
Permeabilization
Yes - Tween-20
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: 1% BSA 1% FBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Any nuclear staining.
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in dog and therefore it may not be suitable for use in this species. Further optimization may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 04 2015