Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mice pancreas with ELA::Myc tumors)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - Tween-20
Specification
mice pancreas with ELA::Myc tumors
Blocking step
Serum as blocking agent for 21 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: 1% BSA 1% FBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Oct4 expression has been reported in pancreatic tumors.
We do not have test any other dilution or blocking solution, but we are very confident about that a higher dilution will remove cytoplasmic signal, probably nonspecific.
More doubts about estromal expression of this protein in this model.
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投稿 Apr 15 2015