Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Cell lysate - whole cell (mES)
Specification
mES
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: Milk 1% in TBS
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: DAKO anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 45 kDa
Exposure
15 minute(s) and 0 second(s)
Additional data
Additional Notes
PyrKinase has been used as loadind control.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 27 2014