Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (primary porcine brain capillary endothelial cells)
Specification
primary porcine brain capillary endothelial cells
Fixative
Aecton/Methanol (3:7)
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen A11034
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Julia Stab
Verified customer
投稿 Oct 14 2011