Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Cow Tissue sections (Oviduct)
Specification
Oviduct
Permeabilization
No
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% BSA in PBST (PBS 0.2% Tween)
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/250
Additional data
Abcam response
new species, image look ok. published PS 17.12.2013
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 17 2013