Application
Western blot
Sample
Cynomolgus Monkey Tissue lysate - whole (Peripheral Blood Mononuclear Cell)
Gel Running Conditions
Reduced Denaturing (Bis-Tris 12%)
Loading amount
20 µg
Specification
Peripheral Blood Mononuclear Cell
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 5°C · Diluent: 5% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat-anti-Rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Super Signal West Femto
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: n/a kDa Non-specific: 220-75 kDa
Positive control
Monkey PBMC cells isolated from Blood
Additional data
Abcam response
Thank you for your review of this previously untested species. We suggest running a sample of a tested species alongside the experimental samples, if possible, to clarify whether the failure is species-specific.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Daniel Tyrrell
Verified customer
投稿 Nov 22 2013