Anti-OBFC1 抗体 (ab89250)
Key features and details
- Mouse polyclonal to OBFC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-OBFC1 antibody
OBFC1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to OBFC1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human OBFC1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human spleen tissue lysate, HeLa cell lysate and OBFC1 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89250の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
ターゲット情報
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機能
Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. -
配列類似性
Belongs to the STN1 family.
Contains 1 OB DNA-binding domain. -
細胞内局在
Nucleus. Chromosome > telomere. - Information by UniProt
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参照データベース
- Entrez Gene: 79991 Human
- Omim: 613128 Human
- SwissProt: Q9H668 Human
- Unigene: 62314 Human
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別名
- AAF 44 antibody
- AAF44 antibody
- Alpha accessory factor 44 antibody
see all
画像
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Anti-OBFC1 antibody (ab89250) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Anti-OBFC1 antibody (ab89250) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 42 kDa
Observed band size: 42 kDa -
All lanes : Anti-OBFC1 antibody (ab89250) at 1 µg/ml
Lane 1 : OBFC1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
データシートおよび資料
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Datasheet download
参考文献 (5)
ab89250 は 5 報の論文で使用されています。
- Lyu X et al. Human CST complex protects stalled replication forks by directly blocking MRE11 degradation of nascent-strand DNA. EMBO J 40:e103654 (2021). PubMed: 33210317
- Chai W et al. The Intrinsically Disordered Region in the Human STN1 OB-Fold Domain Is Important for Protecting Genome Stability. Biology (Basel) 10:N/A (2021). PubMed: 34681076
- Zhang M et al. Mammalian CST averts replication failure by preventing G-quadruplex accumulation. Nucleic Acids Res 47:5243-5259 (2019). PubMed: 30976812
- Wang F et al. Human CST has independent functions during telomere duplex replication and C-strand fill-in. Cell Rep 2:1096-103 (2012). PubMed: 23142664
- Stewart JA et al. Human CST promotes telomere duplex replication and general replication restart after fork stalling. EMBO J 31:3537-49 (2012). PubMed: 22863775