Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Submitted image is taken in the same area as the image submitted for my mouse IHC-P abreview, for this antibody.
Rat shows a similar pattern and distribution of nuclear positivity to that seen in mouse brain
Abcam response
Thank you for your valuable feedback. your data suggests that ab41917 may not be suitable to detect Nurr1 in rat tissue under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Jan 04 2016