Application
Western blot
Sample
Human Cell lysate - whole cell (macrophage)
Loading amount
200 µg
Specification
macrophage
Treatment
20uM compound X and IP perfomred
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: HRP rabbit anti-mouse
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 75 kDa
Additional data
Additional Notes
lane 1 control non-treated sample and IP with Nurr1 antibody
lane 2 compound X treated sample and IP with Nurr1 antibody
I do not expect change expression level of Nurr1 after treatment. It only showed activatio of downstream target.
72kD Nurr1
50kD heavy chain IgG
lane 2 compound X treated sample and IP with Nurr1 antibody
I do not expect change expression level of Nurr1 after treatment. It only showed activatio of downstream target.
72kD Nurr1
50kD heavy chain IgG
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 13 2012