Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cell)
Loading amount
20 µg
Specification
HeLa cell
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: mouse IgG-whole molecule (purified)
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 180 kDa
Additional data
Additional Notes
discount code: 41801
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 21 2013