Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Hela Cells)
Total protein in input
200000 µg
Specification
Hela Cells
Immuno-precipitation step
Protein G
Other product details
Concentration
2 µl/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Western Blot antibody
Name
Non-Abcam antibody was used: anti-GFP
Host species: Rat
Clonality: Polyclonal
Conjugation: Agarose
Host species: Rat
Clonality: Polyclonal
Conjugation: Agarose
Dilution
1/5000
Additional data
Additional Notes
Cells were overexpressed with GFP tagged Nucleostemin
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投稿 Oct 21 2011