Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (DLD1 Cells)
Permeabilization
Yes - 0.5% TritonX-100
Specification
DLD1 Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 1X TBST
Dilution
1/5000
Additional data
Additional Notes
Perfect antibody for Nuclear pore complex staining. Works best for IFA at 1:5000 dilution.
Key steps:
1. Perform CSK digestion before Paraformaldehyde fixation (to digest cytoplasm).
2. 10 min PFA fixation
3. 30 min blocking in 1% BSA
4. 1 hour 30 min incubation at RT in a moist chamber.
5. 1 Wash with 1X PBST followed by 2 washes with 1X PBS at RT.
6. Secondary with Goat Anti-Mouse IgG Alexa 488.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 24 2017