Application
Western blot
Sample
Human Cell lysate - nuclear (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Gel Running Conditions
Reduced Denaturing (4-12% BisTris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: ECL Donkey anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
Positive control
nuclear extracts
Additional data
Additional Notes
Due to great specificity, I use this antibody as loading control for nuclear extracts.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ioannis Gavvovidis
Verified customer
投稿 Dec 03 2010