Application
Western blot
Sample
Human Cell lysate - nuclear (Hela)
Loading amount
60 µg
Specification
Hela
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk in TBS with 0.1%Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti Mouse IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 11 2009