Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell (DDY1810)
Loading amount
0.5 µg
Specification
DDY1810
Gel Running Conditions
Reduced Denaturing (4-12% NUPage gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 3% Milk in TBS tween
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse IgG (whole molecule)-peroxidase produce
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/30000
Detection
Detection method
RCL+
Exposure
45 second(s)
Bands
Specific: 66 KDa kDa
Positive control
Wild type yeast DDY1810
Negative control
NSR1 knock-out in DDY1810
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 28 2010