Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (Spinal cord lysate)
Specification
Spinal cord lysate
Blocking step
Licor Blocking buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IRdye680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Dilution
1/15000
Detection
Detection method
Scan on Odyssey CFx from Licor
Bands
Specific: 58, 68 kDa Non-specific: 40, 80 kDa
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
Band very faint in many samples probably due to the low expression levels of the protein in unstimulated cells. Other cell lines show only one specific band at 55-58kDa without all the other extra bands.
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投稿 Jan 14 2015