Application
Western blot
Sample
Mouse Cell lysate - whole cell (Myeloma)
Loading amount
30 µg
Specification
Myeloma
Treatment
1.25 uM RITA for 6 hrs
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: BSA
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 11 kDa Non-specific: 23, 45, 88 kDa
Additional data
PubMed ID
21062913: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Manujendra Valued Customer
Verified customer
投稿 Jan 07 2011