Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Permeabilization
Yes - Triton X-100 0.5%
Specification
Brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Serum 5%
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Vector horse anti rabbit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
40X image. Fixed in 4% PFA for 10 minutes and counterstained with hematoxylin with TBS washes between every step. Very strong antibody as the DAB developed immediately with a bit of background despite the negative control showing no development.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 28 2012