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Product name
Anti-Notch 2 intracellular domain antibody
Notch 2 intracellular domain 抗体 (2件) 一覧
Description
Rabbit polyclonal to Notch 2 intracellular domain
Specificity
When tested on human cells overexpressing active Notch 2, this antibody detects a band at about 110kD that represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. However, multiple other non-specific bands were also seen in the same experiment.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Peptide: C-NAAERTPSHSGHLQ aa 2396-2409 (the total protein is 2471 aa).
C-NAAERTPSHSGHLQ
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.02% sodium azide
Purity
Whole antiserum
一次抗体 notes
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Cell Cycle >> Cell differentiation
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - Notch 2 intracellular domain antibody (ab8927)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/400. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Target
Relevance
Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Cellular localization
Cell membrane; Single-pass type I membrane protein. NICD: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
Alternative names
- AGS 2 antibody
- AGS2 antibody
- hN2 antibody
see all
Database links
- Entrez Gene: 4853 Human
- Omim: 600275 Human
- SwissProt: Q04721 Human
- Unigene: 487360 Human
Anti-Notch 2 intracellular domain antibody images:
Western blot - Notch 2 intracellular domain antibody (ab8927)
Predicted band size : 110 kDa
Western Blot analysis for Notch 2 expression performed on 100
Lanes1: 24 hours after transfection.
Lane 2: 48 hours after transfection.
Lane 3: 72 hours after transfection.
Lane 4: Control cells 24 hours after transfection.
Lane 5: Control cells 48 hours after transfection.
Lane 6: Control cells 72 hours after transfection.
The band at about 110kD represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band.
Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and hybridized with ab8927 rabbit polyclonal antibody specific for Notch 2 at a 1:400 dilution.
ECL was used as visualization method.
References for Anti-Notch 2 intracellular domain antibody (ab8927)
This product has been referenced in:
- Chen CD et al. Visualization of APP dimerization and APP-Notch2 heterodimerization in living cells using bimolecular fluorescence complementation. J Neurochem 97:30-43 (2006).Read more (PubMed: 16515557) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"