Normal Donkey Serum (ab7475)
製品の概要
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製品名
Normal Donkey Serum
Donkey Serum ihc 試薬 製品一覧 -
由来種
Donkey -
アプリケーション
適用あり: IHC (PFA fixed), Blocking, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
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特記事項
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
製品の特性
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製品の状態
Liquid -
保存方法
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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研究分野
関連製品
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Related Products
- Normal Donkey serum (sterile) (ab138579)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)
- Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150105)
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
- Donkey Anti-Goat IgG H&L (HRP) (ab97110)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC (PFA fixed) |
Use at an assay dependent dilution.
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Blocking |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution.
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特記事項 |
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IHC (PFA fixed)
Use at an assay dependent dilution. |
Blocking
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent dilution. PubMed: 17597760Use at 10% dilution. |
画像
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The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (150)
ab7475 は 150 報の論文で使用されています。
- Alvarez KLF et al. Protocol to assess the effects of dysfunctional human vascular smooth muscle cells on other brain cells using in vitro models of Alzheimer's disease. STAR Protoc 3:101149 (2022). PubMed: 35141568
- Privat-Maldonado A et al. Cold Atmospheric Plasma Does Not Affect Stellate Cells Phenotype in Pancreatic Cancer Tissue in Ovo. Int J Mol Sci 23:N/A (2022). PubMed: 35216069
- Zywitza V et al. Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Sci Rep 12:3100 (2022). PubMed: 35260583
- Nemeth DP et al. Dynamic Interleukin-1 Receptor Type 1 Signaling Mediates Microglia-Vasculature Interactions Following Repeated Systemic LPS. J Inflamm Res 15:1575-1590 (2022). PubMed: 35282272
- Ding H et al. Fisetin ameliorates cognitive impairment by activating mitophagy and suppressing neuroinflammation in rats with sepsis-associated encephalopathy. CNS Neurosci Ther 28:247-258 (2022). PubMed: 34837343