Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Mouse Cell lysate - whole cell (Mouse cardiac fibroblasts)
Specification
Mouse cardiac fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL
Bands
Specific: 50, 52 kDa
Exposure
15 second(s)
Additional data
Additional Notes
This antibody gave 2 specific bands around 50 kDa that correspond to NogoB1 and NogoB2 isoforms.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 29 2013