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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Overview
Product name
Anti-Noelin antibody
Noelin 抗体 (4件) 一覧
Description
Rabbit polyclonal to Noelin
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Chicken, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Noelin.
(Peptide available as ab91078.)
Positive control
This antibody gave a positive signal in Mouse Liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
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Western blot - Noelin antibody (ab35120)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab35120 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Target
Function
Seems to play an important role in regulating the production of neural crest cells by the neural tube.
Sequence similarities
Contains 1 olfactomedin-like domain.
Cellular localization
Endoplasmic reticulum lumen.
Target information above from: UniProt accessionQ99784
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AMY antibody
- D2Sut1e antibody
- Neuronal olfactomedin-related ER localized protein antibody
see all
Database links
- Entrez Gene: 395535 Chicken
- Entrez Gene: 10439 Human
- Entrez Gene: 56177 Mouse
- Entrez Gene: 93667 Rat
- Omim: 605366 Human
- SwissProt: Q9IAK4 Chicken
- SwissProt: Q99784 Human
- SwissProt: O88998 Mouse
see all
Anti-Noelin antibody images:
Western blot - Noelin antibody (ab35120)
Anti-Noelin antibody (ab35120) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Noelin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Noelin antibody (ab35120)
ab35120 has not yet been referenced specifically in any publications.
Publishing research using ab35120? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"