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Western blot - Noelin antibody (ab35120)

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Product Name
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Noelin antibody
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Noelin 抗体 (3件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Noelin
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Noelin. (Peptide available as ab91078.)
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Reacts with
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Ms
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Predicted to react
(species key)
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Hu, Rat, Chk (Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Mouse Liver tissue lysate.
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Cellular localization
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Endoplasmic reticulum lumen.
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Research areas
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Stem Cells >> Neural Stem Cells >> Intracellular Neuroscience >> Neurology process >> Neurogenesis Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
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Relevance
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Noelins belong to the olfactomedin family, which is comprised of extracellular proteins with proposed roles in neural and neural crest development.
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Noelin antibody (ab35120)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 Noelin antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for Noelin antibody images, references, products related to ab35120 and other tools.
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Noelin antibody images:
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Western blot - Noelin antibody (ab35120)
Noelin antibody (ab35120) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa Observed band size : 60 kDa (why is the actual band size different from the predicted?) Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Noelin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Search PubMed (MEDLINE) for references to Noelin
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Noelin antibody - more information
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Customer reviews (feedback) regarding Noelin antibody |
Customer FAQs regarding Noelin antibody |
Protocols for Noelin antibody |
Price and availability of products related to Noelin antibody |
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Noelin antibody - related products:
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Noelin antibody - other tools:
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Contact Abcam with a Technical Enquiry about Noelin antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"