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Western blot - Noelin antibody (ab35120)
Western blot - Noelin antibody (ab35120)
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Product Name 

Noelin antibody

 

Noelin 抗体 (3件) 一覧

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Noelin

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Noelin.

(Peptide available as ab91078.)

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Rat, Chk
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in Mouse Liver tissue lysate.

Cellular localization 

Endoplasmic reticulum lumen.

Research areas 

Stem Cells >> Neural Stem Cells >> Intracellular
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other

Relevance 

Noelins belong to the olfactomedin family, which is comprised of extracellular proteins with proposed roles in neural and neural crest development.

 

Noelin antibody (ab35120)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

395535    (Chicken)
10439    (Human)
56177    (Mouse)
93667    (Rat)

Omim

    

605366    (Human)

SwissProt

    

Q9IAK4    (Chicken)
Q99784    (Human)
O88998    (Mouse)
Q62609    (Rat)

Unigene

    

522484    (Human)
11005    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

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See below for Noelin antibody images, references, products related to ab35120 and other tools.

 

Noelin antibody images:

Product image  Western blot - Noelin antibody (ab35120)

Western blot - Noelin antibody (ab35120)

Noelin antibody (ab35120) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 55 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 8 minutes

Noelin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

 
 

Search PubMed (MEDLINE) for references to Noelin

 

Noelin antibody - more information

Customer reviews (feedback) regarding Noelin antibody

Customer FAQs regarding Noelin antibody

Protocols for Noelin antibody

Price and availability of products related to Noelin antibody

 

Noelin antibody - related products:

Products for Western blot

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Lysates

 

Immunizing Peptide for Noelin antibody

ab91078

  

Noelin peptide

 

Compatible Secondaries for Noelin antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal Secondary Antibody to Rabbit IgG - H&L

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Noelin Antibodies

See all Noelin antibodies (3)...

 

Noelin antibody - other tools:

Contact Abcam with a Technical Enquiry about Noelin antibody

 

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