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ab91078 |
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Anti-Noelin antibody (ab35120) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Noelin precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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