PE Anti-Neutrophil 抗体 [7/4] (ab105157)
Key features and details
- PE Rat monoclonal [7/4] to Neutrophil
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
PE Anti-Neutrophil antibody [7/4]
Neutrophil 一次抗体 製品一覧 -
製品の詳細
PE Rat monoclonal [7/4] to Neutrophil -
由来種
Rat -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse Neutrophil. Cultured mouse bone marrow cells.
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エピトープ
Recognizes the Ly-6B.2 antigen. -
特記事項
ab105157 recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab105157 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
7/4 -
ミエローマ
Y3/Ag1.2.3 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105157の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 10µl for 106 cells. ab134674 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor -
参照データベース
- Entrez Gene: 110453 Mouse
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別名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab105157 は 5 報の論文で使用されています。
- Cai Z et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration. Cell Res N/A:N/A (2016). PubMed: 27444869
- Rosas M et al. The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169-80 (2010). PubMed: 20400676
- Locke LW et al. A novel neutrophil-specific PET imaging agent: cFLFLFK-PEG-64Cu. J Nucl Med 50:790-7 (2009). PubMed: 19372473
- Word RA et al. Transgene insertion on mouse chromosome 6 impairs function of the uterine cervix and causes failure of parturition. Biol Reprod 73:1046-56 (2005). PubMed: 16034000
- Hirsch S & Gordon S Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18:229-39 (1983). PubMed: 6618532