Anti-Neuroserpin 抗体 (ab33077)
Key features and details
- Rabbit polyclonal to Neuroserpin
- Suitable for: WB, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neuroserpin antibody
Neuroserpin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neuroserpin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-FoFrmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human -
免疫原
Synthetic peptide corresponding to Rat Neuroserpin aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab36619) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
ICC/IF | ||
IHC-FoFr | (1) |
特記事項 |
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IHC-FoFr: Use at an assay dependent concentration.
WB: 1/250. Detects bands of approximately 46 and 55 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. -
組織特異性
Predominantly expressed in the brain. -
関連疾患
Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. -
配列類似性
Belongs to the serpin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5274 Human
- Entrez Gene: 20713 Mouse
- Entrez Gene: 116459 Rat
- Omim: 602445 Human
- SwissProt: Q99574 Human
- SwissProt: O35684 Mouse
- SwissProt: Q9JLD2 Rat
- Unigene: 478153 Human
see all -
別名
- DKFZp781N13156 antibody
- Neuroserpin antibody
- NEUS_HUMAN antibody
see all
画像
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All lanes : Anti-Neuroserpin antibody (ab33077) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Neuroserpin contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 55 kDa. The band size observed is also comparable to molecular weights seen with other commercially available antibodies to Neuroserpin. -
ICC/IF image of ab33077 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab33077, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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All lanes : Anti-Neuroserpin antibody (ab33077) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46,55 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab33077 は 6 報の論文で使用されています。
- Higgins NR et al. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Brain Pathol 31:e12948 (2021). PubMed: 33780087
- Adorjan I et al. Neuroserpin expression during human brain development and in adult brain revealed by immunohistochemistry and single cell RNA sequencing. J Anat N/A:N/A (2019). PubMed: 30644551
- Li W et al. Neuroprotective effect of neuroserpin in non-tPA-induced intracerebral hemorrhage mouse models. BMC Neurol 17:196 (2017). WB ; Mouse . PubMed: 29115923
- Kondo S et al. Secretory function in subplate neurons during cortical development. Front Neurosci 9:100 (2015). Mouse . PubMed: 25859180
- Roet KC et al. A multilevel screening strategy defines a molecular fingerprint of proregenerative olfactory ensheathing cells and identifies SCARB2, a protein that improves regenerative sprouting of injured sensory spinal axons. J Neurosci 33:11116-35 (2013). PubMed: 23825416
- Wu J et al. Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death independently of tissue-type plasminogen activator inhibition. Am J Pathol 177:2576-84 (2010). IHC-FoFr ; Mouse . PubMed: 20864675