Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Recombinant protein (neuropeptide Y)
Specification
neuropeptide Y
Blocking step
PVP as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit, sc-2204
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 10 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
I am currently not able to give you a picture of my western blot.
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投稿 Jun 24 2013