Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3 Triton X-100, though not necessary
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 22°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
Additional Notes
The images came from same slice and same area (KF, a part of LPB
on mouse brain atlas). Scale bar is 200 um on left image and 20 um on right image. Blue color for DNA staining (DAPI), while red for NPS.
on mouse brain atlas). Scale bar is 200 um on left image and 20 um on right image. Blue color for DNA staining (DAPI), while red for NPS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Xiaobin Liu
Verified customer
投稿 Jul 14 2009