Anti-Neuronatin 抗体 (ab27266)
Key features and details
- Rabbit polyclonal to Neuronatin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Neuronatin antibody
Neuronatin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neuronatin -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27266の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
Abcam recommends using 1-3% Milk as the blocking agent. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). Abcam recommends using 1-3% Milk as the blocking agent. |
ターゲット情報
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機能
May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels. -
配列類似性
Belongs to the neuronatin family. -
発生段階
Abundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood. - Information by UniProt
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参照データベース
- Entrez Gene: 4826 Human
- Entrez Gene: 18111 Mouse
- Entrez Gene: 94270 Rat
- Omim: 603106 Human
- SwissProt: Q16517 Human
- SwissProt: Q61979 Mouse
- SwissProt: Q62649 Rat
- Unigene: 504703 Human
see all -
別名
- Neuronatin antibody
- NNAT antibody
- NNAT_HUMAN antibody
see all
画像
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Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa -
Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (blocked with 3% Milk) at 50 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 12 minutes -
Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 70-75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab27266 は 19 報の論文で使用されています。
- Zou HY et al. Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice. J Clin Invest 132:N/A (2022). PubMed: 35426376
- Sinha P et al. Trehalose Ameliorates Seizure Susceptibility in Lafora Disease Mouse Models by Suppressing Neuroinflammation and Endoplasmic Reticulum Stress. Mol Neurobiol 58:1088-1101 (2021). PubMed: 33094475
- Saeed H et al. Aberrant epigenetic silencing of neuronatin is a frequent event in human osteosarcoma. Oncotarget 11:1876-1893 (2020). PubMed: 32499872
- Kanno N et al. Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas. J Histochem Cytochem 67:495-509 (2019). PubMed: 30869556
- Vatsa N et al. Down-Regulation of miRNA-708 Promotes Aberrant Calcium Signaling by Targeting Neuronatin in a Mouse Model of Angelman Syndrome. Front Mol Neurosci 12:35 (2019). PubMed: 30814928