Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/400
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Life Technologies Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Immunofluorescence within somatosensory cortex of rat brain (10 um section), counter-stained with DAPI. Produces the appropriate punctate staining, though the antibody is more sensitive than most to freeze-thaw cycles.
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投稿 Dec 17 2015