Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Other product details
Dilution
1/300
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
This antibody produced a beautiful staining in the rat spinal cord. It produced a punctutated staining aroung spinal cells, that look like neurons. The staining seems to be on the membrane of the cells, including on their dendrites. This staining is consistent with the localisation of this cell adhesion molecule, linked to PDZ, on one hand, and with the localisation of its mRNA described by the ‘Allen Brain Institute’, on an other hand.
Image A shows the staining observed at the level of the lateral part of the dorsal horn, showing stained lamina II neurons (arrows). Image B shows the staining observed lamina X at the level of spinal neurons isolated and located in each site of the central canal (arrows).
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique.
Image A shows the staining observed at the level of the lateral part of the dorsal horn, showing stained lamina II neurons (arrows). Image B shows the staining observed lamina X at the level of spinal neurons isolated and located in each site of the central canal (arrows).
The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the tissues were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Sep 06 2010