Anti-Neurogenin 1/NEUROG1 抗体 (ab66498)
Key features and details
- Rabbit polyclonal to Neurogenin 1/NEUROG1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Neurogenin 1/NEUROG1 antibody
Neurogenin 1/NEUROG1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Neurogenin 1/NEUROG1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human Neurogenin 1/NEUROG1 (C terminal).
Database link: Q92886 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66498の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/1000.
|
|
ICC/IF |
1/1000.
|
特記事項 |
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IHC-P
1/1000. |
ICC/IF
1/1000. |
ターゲット情報
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機能
Appears to mediate neuronal differentiation. -
組織特異性
Expression restricted to the embryonic nervous system. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4762 Human
- Entrez Gene: 18014 Mouse
- Omim: 601726 Human
- SwissProt: Q92886 Human
- SwissProt: P70660 Mouse
- Unigene: 248149 Human
- Unigene: 266665 Mouse
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別名
- AKA antibody
- Ath4c antibody
- bHLHa6 antibody
see all
画像
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ICC/IF image of ab66498 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66498, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Ab66498 staining human normal liver. Staining is localised to the nucleus.
Left panel: with primary antibody diluted 1:1000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab66498 は 5 報の論文で使用されています。
- Zhang L et al. Loss of ZC4H2 and RNF220 Inhibits Neural Stem Cell Proliferation and Promotes Neuronal Differentiation. Cells 9:N/A (2020). PubMed: 32630355
- Huang M et al. Paraquat affects the differentiation of neural stem cells and impairs the function of vascular endothelial cells: a study of molecular mechanism. Environ Toxicol 34:548-555 (2019). PubMed: 30698896
- Han S et al. A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis. Development 145:N/A (2018). PubMed: 30201687
- Zhang X et al. Impact of siRNA targeting of ß-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage. Mol Med Rep 14:3595-601 (2016). WB ; Rat . PubMed: 27573468
- Blanchard JW et al. Selective conversion of fibroblasts into peripheral sensory neurons. Nat Neurosci 18:25-35 (2015). ICC ; Mouse . PubMed: 25420069