Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole 3 day post fertilization zebrafish embryos)
Loading amount
50 µg
Specification
whole 3 day post fertilization zebrafish embryos
Gel Running Conditions
Reduced Denaturing (3-8% TA)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Cell Signaling Anti-rabbit IgG, HRP-linked Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Cell Signaling 20X LumiGLO® Reagent and
Exposure
30 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: ~300 kDa
Positive control
HEK 239T cells, mouse whole embryo lysate (E10.5)
Negative control
Nf1-/- mouse whole embryo lysate (E10.5)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 09 2009