Application
ChIP
Detection step
Other
Sample
Mouse Tissue lysate - whole (Cerebral cortex tissue from postnatal day 0 mouse)
Specification
Cerebral cortex tissue from postnatal day 0 mouse
Negative control
ChIP performed with unrelated antibody (anti-GFP antibody).
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
Known target of NeuroD2, which is the promoter regions of Nhlh2 gene.
Primer sequence for PCR was:
Nhlh2_F CTCACGAACTTCACCCGCAC
Nhlh2_R AAATTGACTCCTCGGCCCTC
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Additional data
Abcam response
We are very happy that this antibody provided good results for this customer.
Since the customer obtained positive results in ChiP, we decided to re-test this antibody in IP to determine its suitability for this application.
The lab re-tested the same lot used by the customer in his ChiP assay. Unfortunately, the results obtained were negative therefore we still do not recommend this antibody in IP.
Please feel free to contact our Scientific Support team if you have some feedback you would like to share and/or if you have any question in this matter.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 18 2014