Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization
No
Specification
brain
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine anti-mouse/rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
various pretreatment regimes tested. EDTA gave best signal to background.
Procedure automated on Leica Bond RX
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投稿 Aug 25 2015