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Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Overview
Product name
Anti-NeuroD1 antibody
NeuroD1 抗体 (10件) 一覧
Description
Mouse monoclonal to NeuroD1
Tested applications
IHC-Fr, IHC-P, WB, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human, Apteronotus leptorhynchus
Predicted to work with
Rat, Sheep, Cow
Immunogen
Recombinant fragment with tag: QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT , corresponding to amino acids 201-300 of Human NeuroD1
QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT
Positive control
IMR-32 (human neuroblastoma) whole cell lysate and human ovary, clear cell carcinoma tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab60704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: Use a concentration of 3 µg/ml
WB: Use at an assay dependent dilution. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
ELISA: Use at an assay dependent dilution. (The detection limit for ab60704 is approximately 0.03ng/ml as a capture antibody.)
ICC/IF: Use at an assay dependent concentration.
Target
Function
Differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex. Transcriptional activator. Binds to the insulin gene E-box.
Involvement in disease
Defects in NEUROD1 are the cause of maturity-onset diabetes of the young type 6 (MODY6) [MIM:606394]. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation; which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335; which promotes dendritic growth.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ13562
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- atonal antibody
- basic helix loop helix transcription factor antibody
- BETA 2 antibody
see all
Database links
- Entrez Gene: 4760 Human
- Entrez Gene: 18012 Mouse
- Entrez Gene: 29458 Rat
- Omim: 601724 Human
- SwissProt: Q13562 Human
- SwissProt: Q60867 Mouse
- SwissProt: Q64289 Rat
- Unigene: 574626 Human
see all
Anti-NeuroD1 antibody images:
Western blot - NeuroD1 antibody (ab60704)
Anti-NeuroD1 antibody (ab60704) + IMR-32 whole cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
developed using the ECL technique
Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NeuroD1 antibody (ab60704)
ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - NeuroD1 antibody (ab60704)
Immunofluorescence image of NeuroD1 (ab60704) on D3 mouse embryonic stem cells after 14 days of differentiation. The cells were fixed in paraformaldehyde and permeabilized in block solution. In this image you can see NeuroD1 positive staining with ab60704 in green.
Image taken from an anonymous abreview
Immunohistochemistry (Frozen sections) - NeuroD1 antibody (ab60704)
ab60704 staining NeuroD1 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton in TBS for 30 minutes and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/500) for 15 hours at 4°C. An undiluted Alexa Fluor®633-conjugated Goat anti-mouse monoclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Ellie Tuck
References for Anti-NeuroD1 antibody (ab60704)
This product has been referenced in:
- Albanito Let al. c-Jun is essential for the induction of Il-1ß gene expression in in vitro activated Bergmann glial cells. Glia : (2011).Read more (PubMed: 21948257) »
- Kroehne Vet al. Regeneration of the adult zebrafish brain from neurogenic radial glia-type progenitors. Development 138:4831-41 (2011).Read more (PubMed: 22007133) »
See all 3 publications for this product
Publishing research using ab60704? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"