Anti-NeuroD1 antibody (ab60704)

Western blot - NeuroD1 antibody (ab60704)

Anti-NeuroD1 antibody (ab60704) + IMR-32 whole cell lysate at 50 µg

Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
developed using the ECL technique

Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NeuroD1 antibody (ab60704)

ab60704 at 3ug/ml staining NeuroD1 in human ovary, clear cell carcinoma by Immunohistochemistry, Formalin-fixed Paraffin-embedded tissue.

Immunocytochemistry/ Immunofluorescence - NeuroD1 antibody (ab60704)

Immunofluorescence image of NeuroD1 (ab60704) on D3 mouse embryonic stem cells after 14 days of differentiation. The cells were fixed in paraformaldehyde and permeabilized in block solution. In this image you can see NeuroD1 positive staining with ab60704 in green.

Image taken from an anonymous abreview

Immunohistochemistry (Frozen sections) - NeuroD1 antibody (ab60704)

ab60704 staining NeuroD1 in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton in TBS for 30 minutes and blocked with 5% serum for 1 hour at 20ºC. Samples were incubated with primary antibody (1/500) for 15 hours at 4ºC. An undiluted Alexa Fluor^®633-conjugated Goat anti-mouse monoclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Ellie Tuck

Flow Cytometry-Anti-NeuroD1 antibody(ab60704)

Overlay histogram showing SHSY-5Y cells stained with ab60704 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60704, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.