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Immunocytochemistry - NeuroD1 antibody (ab16508)
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Western blot - NeuroD1 antibody (ab16508)
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Product Name
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NeuroD1 antibody
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NeuroD1 抗体 (7件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to NeuroD1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NeuroD1.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat
(Due to sequence homology)
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Specificity
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ab16508 is raised against an immunogen specific for human, rat and mouse NeuroD1. The immunizing peptide also has 100% identity (based on a BLAST search) with mouse and rat NeuroD2, human NeuroD4, mouse and human NeuroD6 and rat MIST1.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at an assay dependent concentration.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-Fr: Use at an assay dependent dilution. (Abreview: 4163)
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20531234)
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 38-43 kDa (predicted molecular weight: 38-43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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mouse lung, human brain, fixed cultures of murine neurones differentiated for 3 days from murine neocortical precursor cells (E14).
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Metabolism
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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NeuroD1 (Neurogenic Differentiation factor 1, also known as BETA2, beta-cell E-box transactivator 2) is a 40kDa protein, a member of the bHLH family. NeuroD1 is a homolog of the Drosophila atonal gene. NeuroD1 is widely expressed during development in the mammalian brain and pancreas where it acts as a transcriptional activator, and binds to insulin gene E-box. NeuroD1 has also been reported to be a differentiation factor in neurogenesis. Changes in NeuroD1 gene expression have been linked to diabetes mellitus type II and maturity-onset diabetes of the young type VI. NeuroD1 expression can be induced by glucose in beta cells.
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NeuroD1 antibody (ab16508)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 NeuroD1 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for NeuroD1 antibody images, references, products related to ab16508 and other tools.
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NeuroD1 antibody images:
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 Immunocytochemistry - NeuroD1 antibody (ab16508)
[A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging).Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed. [B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody. [C] blue = TO-PRO-3 (nuclear) staining [D] Overlay of A, B and C demonstrates localisation of NeuroD1 to the cell bodies of these immature neurons. Randal Moldrich, CNRS UMR7637, ESPCI, France
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Western blot - NeuroD1 antibody (ab16508)
Lane 1 : Marker
Lanes 2 - 3 : NeuroD1 antibody (ab16508) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Human) Tissue Lysate (ab7925) at 20 µg
Lane 3 : Brain (Human) Tissue Lysate (ab7925) at 20 µg with NeuroD1 peptide (ab17100) at 1 µg/ml
Secondary
Lanes 2 - 3 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 38-43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~49 kDa,~60 kDa. We are unsure as to the identity of these extra bands.
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References for NeuroD1 antibody (ab16508)
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This product has been used in: (two most recent references)
Puligilla C et al. Sox2 induces neuronal formation in the developing mammalian cochlea. J Neurosci 30:714-22 (2010). IHC-P; Mouse. PubMed: 20071536
Dong Y et al. Role of phosphatase and tensin homolog in the development of the mammalian auditory system. Neuroreport 21:731-5 (2010). IHC-FoFr; Mouse. PubMed: 20531234
See all 3 publication references for this product.
If you publish research using ab16508 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to NeuroD1
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NeuroD1 antibody - more information
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Customer reviews (feedback) regarding NeuroD1 antibody |
Customer FAQs regarding NeuroD1 antibody |
Protocols for NeuroD1 antibody |
Price and availability of products related to NeuroD1 antibody |
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NeuroD1 antibody - related products:
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NeuroD1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about NeuroD1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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