Products:Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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ab17100 |
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ab16508 has been referenced in 6 publications.
Publishing research using ab16508? Please let us know so that we can cite the reference in this datasheet
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[A] green = ab16508 Rabbit polyclonal antibody to NeuroD1 staining (5ug/ml)) in a fixed culture of murine neurons, differentiated for 3 days, from murine neocortical precursor cells (E14). Neurons shown emanated from the site of a neurosphere (top-left corner) which was removed prior to imaging). Alexa Fluor 488 secondary goat anti-rabbit antibody was used for detection (@ 1/400). Strong punctate nuclear and diffuse cytosolic NeuroD1 labelling at is observed.
[B] red = mouse monoclonal anti-MAP (microtubule marker) with goat anti-mouse Alexa Fluor 568 (1/400) secondary antibody.
[C] blue = TO-PRO-3 (nuclear) staining
[D] Overlay of A, B and C demonstrates localisation of NeuroD1 to the cell bodies of these immature neurons.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Lane 1 : Marker
Lanes 2 - 3 : Anti-NeuroD1 antibody (ab16508) at 1 µg/ml
Lane 1 : As above
Lane 2 : Brain (Human) Tissue Lysate (ab7925) at 20 µg
Lane 3 : Brain (Human) Tissue Lysate (ab7925) at 20 µg with
Secondary
Lanes 2 - 3 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 38-43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~49 kDa,~60 kDa. We are unsure as to the identity of these extra bands.
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