Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (bone marrow-mesenchymal stem cell)
Permeabilization
Yes - saponin 0,1%
Specification
bone marrow-mesenchymal stem cell
Blocking step
Protein block serum-free ready to use (DAKO) + saponin 0,1% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Protein block serum-free .. + saponin
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: alexa goat anti mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
This antibody was tested in pig samples using our Abtrial program. The results here indicate that this antibody may not react with pig, or that further optimization is required for use in pig. Please contact Scientific Support with any questions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 23 2016