Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (seedlings)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
100 µg
Treatment
protease inhibitor
Specification
seedlings
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST 3%milk
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Pierce Stabilized Goat anti-Mouse HRP 1858413
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Detection
Detection method
ECL termo femto and pico
Negative control
wildtype
Exposure
12 hour(s), 0 minute(s) and 0 second(s)
Positive control
used anti-NPTII antibody from Fitzgerald which produced bands
Additional data
Abcam response
We would like to thank this customer for his Abreview. We are sorry to hear that ab60018 did not deliver positive results. Despite not being tested in plants material before, we would expect the antibody to work in this sample type.
Since this antibody was bought 5 years prior to the experiment, we must assume that is lost its activity over time. Please be aware that we provide a guarantee for our product for 12 month (www.abcam.com/abpromise)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Nico Dissmeyer
Verified customer
投稿 Aug 03 2015