![Western blot - Ndufs4 antibody [2C7CD4AG3] (ab87399) image](#Ndufs4-Primary-antibodies-ab87399-1.jpg)
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Product name
Anti-Ndufs4 antibody [2C7CD4AG3]
Ndufs4 抗体 (4件) 一覧
Description
Mouse monoclonal [2C7CD4AG3] to Ndufs4
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Recombinant full length protein (Human)
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: HEPES, Sodium chloride
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
2C7CD4AG3
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex I
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - Ndufs4 antibody [2C7CD4AG3] (ab87399)
![Western blot - Ndufs4 antibody [2C7CD4AG3] (ab87399) image](/ps/datasheet/images/87/ab87399/Ndufs4-Primary-antibodies-ab87399-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab87399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Target
Function
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Involvement in disease
Defects in NDUFS4 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
Sequence similarities
Belongs to the complex I NDUFS4 subunit family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionO43181
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- AQDQ antibody
- CI 18 kDa antibody
see all
Database links
- Entrez Gene: 327680 Cow
- Entrez Gene: 4724 Human
- Entrez Gene: 17993 Mouse
- Entrez Gene: 499529 Rat
- Omim: 602694 Human
- SwissProt: Q02375 Cow
- SwissProt: O43181 Human
- SwissProt: Q9CXZ1 Mouse
see all
Anti-Ndufs4 antibody [2C7CD4AG3] images:
Western blot - Ndufs4 antibody [2C7CD4AG3] (ab87399)
All lanes : Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
Lane 1 : Isolated mitochondria from human heart at 15 µg
Lane 2 : Isolated mitochondria from bovine heart at 6 µg
Lane 3 : Isolated mitochondria from rat heart at 30 µg
Lane 4 : Isolated mitochondria from mouse heart at 30 µg
Predicted band size : 20 kDa
Observed band size : 20 kDa
References for Anti-Ndufs4 antibody [2C7CD4AG3] (ab87399)
This product has been referenced in:
- Feng J et al. Phosphoproteome analysis of isoflurane-protected heart mitochondria: phosphorylation of adenine nucleotide translocator-1 on Tyr194 regulates mitochondrial function. Cardiovasc Res 80:20-9 (2008).Read more (PubMed: 18558627) »
- Feng J et al. Tyrosine phosphorylation by Src within the cavity of the adenine nucleotide translocase 1 regulates ADP/ATP exchange in mitochondria. Am J Physiol Cell Physiol 298:C740-8 (2010). WB; Rat.Read more (PubMed: 20007455) »
See all 4 publications for this product
Publishing research using ab87399? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"